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In order to determine yield stability and genotype × environment interaction effects under terminal drought stress and non-stress conditions, 20 promising barley lines were evaluated during 2018 to 2020. Experiments were conducted using randomized complete block design with three replications in eight stations of moderate zone including: Birjand, Yazd and Varamin (terminal drought stress and non-stress conditions), Karaj, Mashhad, Neishabour, Esfahan and Zarghan (non-stress conditions). Combined analysis of variance for yield in non-stress conditions and under terminal drought stress showed significant differences for year × location and genotype (only non-stress conditions) and genotype × location × year interaction effects (both conditions). Mean comparisons of the yield using Duncan Multiple Range test (DMRT) showed that genotypes No.3, 9, 20 and 18 had the highest yield (6.933, 6.859, 6.816 and 6.809 t/ha) in non-stress conditions and genotypes No. 11, 13 and 7 had the highest yield (6.058, 6.003 and 5.656 t/ha) in terminal drought stress conditions among genotypes. Different stability analysis methods were employed to determine the effects of G × E interactions as well as genotype adaptability. Overall, genotypes No.3(EBYTM97-3), 9(EBYTM97-9), 11(EBYTM97-11), 13(EBYTM97-13) and 18(EBYTM97-18) had the highest adaptability among genotypes using different methods of stability. These genotypes were selected as candidates for on-farm trials from which some varieties may be released.Key words: barley, yield stability, adaptation, drought stress, genotype × environment interaction effect and moderate zone
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