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This study was conducted with the aim of evaluating yield and agronomic traits of 41 mutant genotypes, resulted from chemical mutation of Hashemi local rice variety, with control cultivars Hashemi, Anam, TH2 and Gilaneh in an augmented design based on a randomized complete block design with 4 replications, at the Rice Research Institute of Iran, Rasht, in 2019. In this study, agronomic and qualitative characteristics including flowering time, plant height, number of fertile tillers, panicle length, number of filled grains, number of unfilled grains, 100-grain weight, milling recovery, amylose content, mean gelatinization temperature and grain yield were assessed in all genotypes. Based on the results, there were considerable variations among genotypes in terms of flowering time (days to 50% of flowering), plant height, panicle length, grain yield, milling recovery and amylose content, and the differences among genotypes and as well as differences among genotypes with controls in all cases were significant (p≤0.05). Number of days from planting to flowering was less than 83 days in 33% of genotypes. Also, in 43% of lines, plant height was less than 130 cm and 30% of genotypes had yields higher than 5 t/ha. Results showed that mutant genotypes EM4-233 and EM4-233 had highest yields (more than 6 t/ha) and were superior to other genotypes. Regarding earliness, grain quality, yield traits and other desirable agronomic characteristics, mutant genotypes EM4-83, EM4-233, EM4-29, EM4-106 and EM4-294 were selected as superior genotypes. Key words: Ethylemethane solphonate, genetic diversity, mutation, rice
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