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Based on the obtained results, the intestinal tract of the honeybees contained Lactobacillus, Enterococcus, Pediococcus and bacillus species. Majority of the isolates (71%) belonged to geus lactobacillus. According to the molecular analysis (16SrRNA) the isolates were identified as L.casei (1 isolate), L.apis(1 isolate), L.fermentum(1 isolate), L.plantarum(2 isolates), Pediococcus acidilactici(2 isolates), Enterococcus faecium(2 isolates), L.kunkee((1 isolate) and L.rhamnosus(1 isolate). Additonally, the three Bacillus species isolated from honey samples were identified as Bacillus megaterium, Bacillusleichniformisand B.subtilis. The isolates were characterized for their probiotic characteristics in invitro conditions. The tests included pH and bile salt tolerance, antibacterial activity, antibiotic sensitivity, tolerance to intestinal and duodenal conditions, adherence ability to epithelial cells, cell surface hydrophobicity, aggregation and co-aggregation abilities. Results of this study showed that all the isolates were able to tolerate acidic pH (pH 2.5 and 4) and also high concentrations of bile salts (0.5, 0.7 and 1%). Additionally, the tested lactobacillus isolates also possessed the potential to hydrolyze bile acids. The selected seven lactic acid bacteria showed higher percentage of survival in intestinal conditions compared to gastric conditions. While, compared to these strains all three tested bacillus species had higher survival ratios in both intestinal and gastric conditions. The antibacterial activity of the selected strains against selected pathogens confirmed their antibacterial potential. The isolates appeared sensitive to majority ofthe testedantibiotic discs except for vancomycin and nalidix acid to whichthe Lab bacteria very resistant.During aggregation and co-aggregation studies, L.casei, and L.apisshowed highest aggregation ability(p˂0.05). Both these isolates also showed highest cell surface hydrophobicity activities. Compared to Bacillus species, the tested LAB species showed higher adherence ability especially L.casei, E.faeciumand L.apishad the highest adherence to CaCo2 cell lines. Keywords: Honeybees, probiotics, Lactic Acid bacteria, bacillus, Bile salts, Antibiotic resistance, Antibacterial activity, CaCo2 cell adherence, Aggregation and Co-aggregation.
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