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Proxy acetate Acid has a rapid action against micro-organisms and is used as a high-level disinfectant for critical and semi-critical devices. In this study, the effect of homemade Aquastat was investigated on Saprolegniosis, green eggs and its biological effects on rainbow trout larvae and live fish tissue. In addition, the pathological study of its various concentrations on gills and the effects of water disinfection on microbial and fungal agents and its 96-hour Lc50 were also investigated. On the other hand, MIC, MBC and MFC were investigated in In vivo conditions on Streptococcus iniae, Lactococcus garvieae , Yersinia rachery and Saprolegnia parasitica fungi. Meanwhile, all the variables were compared with the antimicrobials including formalin, malachite green. The results of this study showed that Aquastat in rainbow trout propagation conditions in the country have an effective disinfectant effect on inhibiting and killing mentioned bacterial agents and controlling the destructive effects of Saprolegniosis in green eggs of rainbow trout.Keywords: Aquastat, Green egg of Rainbow trout, propagation, Disinfectant, Fungi
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